polyacrylamide gel

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polyacrylamide gel A gel used to separate biological molecules (e.g. proteins of a given range of sizes). It is prepared by mixing a monomer (acrylamide) with a cross-linking agent (N, N'–methylenebisacrylamide) in the presence of a polymerizing agent, and leads to the formation of an insoluble 3-dimensional network of monomer chains, which become hydrated in water. Gels of different pore sizes may be prepared depending upon the relative proportions of the ingredients.